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Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium
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Yalda Basim    , Ghasemali Mohebali , Sahand Jorfi , Ramin Nabizadeh , Mehdi Ahmadi Moghadam , Ata Ghadiri , Nematollah Jaafarzadeh Haghighi Fard |
| Corresponding author:Environmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , Jaafarzadeh-n@ajums.ac.ir |
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Abstract: (3327 Views) |
Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium.
Methods: The bacterial strains present in the contaminated and non-contaminated soils were identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were determined using a gas chromatograph equipped with a flame ionization detector (GC-FID).
Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae (84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial strains was less in oil-contaminated soils and varied significantly between case and control samples. Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of
heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is one of the innovative aspects of this research.
Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency of 28.1% was obtained. |
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Keywords: Oil-contaminated soils, Biodegradation, Bacterial diversity, Total petroleum hydrocarbons, Indigenous bacterial consortium
eprint link: http://eprints.kmu.ac.ir/id/eprint/32727 |
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Full-Text [PDF 531 kb]
(1456 Downloads)
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Type of Study: Original Article |
Subject:
General
Received: 2020/07/10 | Accepted: 2020/05/30 | Published: 2020/08/5
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