Environmental Health Engineering and Management Journal

en Investigation of toxicity of TiO2 nanoparticles on glioblastoma and neuroblastoma as the most widely used nanoparticles in photocatalytic processes تخصصي Special مقاله اصیل Original Article <div style="text-align: justify;">Background: Titanium dioxide (TiO2) nanoparticles (NPs) are among the most important and usable photocatalysts. Recently, the biological properties of these NPs, particularly, its anticancer activity, have been considered. Glioblastoma and neuroblastoma are two fatal brain tumors with a high mortality rate in humans, the hope for treatment of which is weak by the common methods. Methods: In this study, the cytotoxicity effects of TiO2 alone and in combination with ultraviolet A (UVA) irradiation on two different cell lines, neuroblastoma (SH-SY5Y) and glioblastoma U87, were investigated. After administration of 10, 50, 100, and 500 μg/mL TiO2, 0.043 and 1.4 mW/cm2 UVA irradiation, cell viability was investigated after 4, 24, and 48 hours by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. Results: MTT assay and light microscope demonstrated that the effect of TiO2 NPs varied based on the dose of the substance, the impact time, the cell type, and the amount of radiation. In this study, for NPs alone, both toxicity and non-toxicity of the substance were observed. For NPs in the presence of UV, based on the comparison with its status alone and the difference in the viability assay of the two groups, both the photocatalytic and the coating effect of the NPs were observed. Conclusion: According to the results, different concentrations of TiO2 can be used for different purposes. Low concentrations of TiO2 can be used to increase the efficiency of photodynamic therapy and high concentrations of TiO2 can be used to protect the normal cell. This strategy improves the photodynamic therapy and reduces the harmful effects.</div> Titanium, Nanoparticles, UV radiation, Neuroblastoma, Glioblastoma 365 374 http://ehemj.com/browse.php?a_code=A-10-1-274&slc_lang=en&sid=1 Fahimeh Kazemi 100319475328460011330 100319475328460011330 No School of Medicine, Bam University of Medical Sciences, Bam, Iran Marzie Esmaeeli 100319475328460011331 100319475328460011331 No Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran Peyman Mohammadzadehjahani 100319475328460011332 100319475328460011332 No School of Medicine, Bam University of Medical Sciences, Bam, Iran Mahnaz Amiri 100319475328460011333 100319475328460011333 No Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran Parisa Vosough 100319475328460011334 100319475328460011334 No Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran Meysam Ahmadi-Zeidabadi meysamcell@yahoo 100319475328460011335 100319475328460011335 Yes Corresponding author: Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran