Environmental Health Engineering and Management Journal

en Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium عمومى General مقاله اصیل Original Article <div style="text-align: justify;">Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains present in the contaminated and non-contaminated soils were identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were determined using a gas chromatograph equipped with a flame ionization detector (GC-FID). Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae (84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial strains was less in oil-contaminated soils and varied significantly between case and control samples. Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is one of the innovative aspects of this research. Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency of 28.1% was obtained.</div> Oil-contaminated soils, Biodegradation, Bacterial diversity, Total petroleum hydrocarbons, Indigenous bacterial consortium 127 133 http://ehemj.com/browse.php?a_code=A-10-1-168&slc_lang=en&sid=1 Yalda Basim yaldabasim@gmail.com 10031947532846006686 10031947532846006686 No Environmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Ghasemali Mohebali mohebaligh@ripi.ir 10031947532846006687 10031947532846006687 No Microbiology and Biotechnology Research Group, Research Institute of Petroleum Industry, Tehran, Iran Sahand Jorfi Jorfi-s@ajums.ac.ir 10031947532846006688 10031947532846006688 No Environmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Ramin Nabizadeh rnabizadeh@gmail.com 10031947532846006689 10031947532846006689 No Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mehdi Ahmadi Moghadam ahmadi-m@ajums.ac.ir 10031947532846006690 10031947532846006690 No Environmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Ata Ghadiri ghadiri-a@ajums.ac.ir 10031947532846006691 10031947532846006691 No Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Nematollah Jaafarzadeh Haghighi Fard Jaafarzadeh-n@ajums.ac.ir 10031947532846006692 10031947532846006692 Yes Corresponding author:Environmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran