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:: Volume 8, Issue 4 (Autumn 2021) ::
Environ. Health Eng. Manag. 2021, 8(4): 303-308 Back to browse issues page
Frequency and antibiotic resistance of Helicobacter pullorum among exposed and non-exposed population
Hosein Akhlaghi , Seyed Hesamodin Emadi Chashmi , Ashkan Jebelli Javan
Corresponding author:Department of Clinical Science, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran , heamemadi@semnan.ac.ir
Abstract:   (625 Views)
Background: Helicobacter pullorum can infect the intestinal tracts of both humans and avian species. This study aimed to assess the frequency and antibiotic resistance of H. pullorum isolated from workers in the poultry slaughterhouses, farms, and markets as exposed population and healthy people who referred to the hospital as non-exposed population by culture method and polymerase chain reaction (PCR) test.
Methods: Two hundred healthy individuals, including 100 individuals from exposed population and 100 from non-exposed population were selected in Semnan. Fresh stool samples were examined by conventional culture method and biochemical tests. PCR test with 16S rRNA gene was employed to confirm the H. pullorum isolates. Antibiotic resistance test was done using the disk diffusion method and various antimicrobial agents.
Results: Generally, 17 (17%) samples from exposed population and 12 (12%) samples from non-exposed population were H. pullorum positive by culture method and biochemical tests. However, PCR test could confirm 10 (10%) and 7 (7%) samples from exposed and non-exposed populations, respectively. Therefore, the frequency of H. pullorum was determined to be 9.5%. Antibiotic resistance test could reveal that most of the isolates were resistant to ciprofloxacin (84.2%), whereas resistance to colistin and fosfomycin was found to be 15.8%.
Conclusion: The present study illustrated that H. pullorum can be present among healthy population with the low frequency rate. Moreover, it was indicated that the frequency of this food-borne pathogen is high in the exposed population. Therefore, there is a high demand for good observation for slaughter hygiene and implementation of routine surveillance in the poultry farms and markets.
Keywords: Helicobacter pullorum, Frequency, Humans, Poultry, Polymerase chain reaction
eprint link: http://eprints.kmu.ac.ir/id/eprint/38738
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Type of Study: Original Article | Subject: General
Received: 2021/11/28 | Accepted: 2021/11/1 | Published: 2021/12/12
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Akhlaghi H, Emadi Chashmi S H, Jebelli Javan A. Frequency and antibiotic resistance of Helicobacter pullorum among exposed and non-exposed population. Environ. Health Eng. Manag.. 2021; 8 (4) :303-308
URL: http://ehemj.com/article-1-852-en.html


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Volume 8, Issue 4 (Autumn 2021) Back to browse issues page
مجله مدیریت و مهندسی بهداشت محیط Environmental Health Engineering And Management Journal
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