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:: Volume 2, Issue 3 (Summer, 2015) ::
Environ. health eng. manag. 2015, 2(3): 141-147 Back to browse issues page
Optimization of culture media for lipid production by Nannochloropsis oculata for Biodiesel production
Mohammad Malakootian , Behnam Hatami, Shidwash Dowlatshahi, Ahmad Rajabizadeh
Environmental Health Engineering Research Center, Department of Environmental Health, Kerman University of Medical Sciences, Kerman, Iran , m.malakotian@gmail.com
Abstract:   (7911 Views)

Background: This study quantified the effect of four popular culture media in a biodiesel production study on the qualitative and quantitative lipid content, dry biomass, and lipid productivity of Nannochloropsis oculata. Methods: Culture of microalgae was done separately in Walne, F/2, Sato, and TMRL media. In the logarithmic and stationary growth phases, biomass production and lipid accumulation of microalgae were measured and the constituents were identified by gas chromatography. Results: N. oculata exhibited the highest rate of cell growth and biomass productivity of 0.2616 day-1 and 2.652 gl-1 in the Walne medium. The highest level of biomass conversion into lipids in TMRL medium revealed a cell dry weight of 37.22%. Walne medium proved to have the most efficient lipid productivity which was 0.1057 gl-1 day-1. The highest amount of triacylglycerol (TAG) was obtained in Sato medium in the stationary growth phase and was 75.25% of the fatty acids. Conclusion: The present study provides a practical benchmark, which allows the introduction of Walne as a suitable culture medium for N. oculata in biodiesel studies.

Keywords: Nannochloropsis oculata, Culture, Medium, Biofuel, Biodiesel, Lipid productivity
eprint link: http://eprints.kmu.ac.ir/id/eprint/22205
Full-Text [PDF 435 kb]   (3229 Downloads)    
Type of Study: Original Article | Subject: General
Received: 2015/09/22 | Accepted: 2015/09/22 | Published: 2015/09/22
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Malakootian M, Hatami B, Dowlatshahi S, Rajabizadeh A. Optimization of culture media for lipid production by Nannochloropsis oculata for Biodiesel production. Environ. health eng. manag.. 2015; 2 (3) :141-147
URL: http://ehemj.com/article-1-92-en.html

Volume 2, Issue 3 (Summer, 2015) Back to browse issues page
مجله مدیریت و مهندسی بهداشت محیط Environmental Health Engineering And Management Journal
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