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:: Volume 9, Issue 3 (Summer 2022) ::
Environ. Health Eng. Manag. 2022, 9(3): 305-310 Back to browse issues page
Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water
Fathollah Gholami-Borujeni * , Hamzeh Rahimi , Seyed Mehdi Sajjadi
Corresponding author: Department of Environmental Health Engineering, Faculty of Health, Mazandaran University of Medical Sciences, Sari, Iran , fa.gholami@mazums.ac.ir
Abstract:   (96 Views)
Background: Helicobacter pylori is classified as a carcinogen, and it is also the most common cause of chronic bacterial infection and peptic ulcers. Approximately 45% of people are infected with the bacterium.
Methods: In this study, the H. pylori genes, CagA and VacA, were investigated in drinking water, using 100 samples (50 samples from the municipal water supply and 50 samples from the effluent of household water treatment devices). DNA was extracted from colonies with a positive heterotrophic plate count (HPC) for use in molecular testing and microbial identification. The polymerase chain reaction (PCR) was used to identify H. pylori.
Results: The study showed that 24% of urban water samples (12% above the World Health Organization [WHO] standards for safe drinking water) and 18% of home water treatment-device samples (4% above the WHO standards) were HPC-positive. The H. pylori genes, CagA and VacA, were identified in 2% of the samples from household water treatment devices and 8% of the municipal water supply samples.
Conclusion: The study findings show that H. pylori may be transmitted in drinking water. However, there is currently no strong evidence that the bacteria can survive after the disinfection process in the water supply system. Therefore, the health risks of this bacterium in drinking water are still unknown.
Keywords: Helicobacter pylori, Drinking water, Polymerase chain reaction, Water supply, Water purification
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Type of Study: Original Article | Subject: General
Received: 2022/09/11 | Accepted: 2022/07/1 | Published: 2022/09/19
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Ethics code: IR.MAZUMS.REC.1397.3011

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Gholami-Borujeni F, Rahimi H, Sajjadi S M. Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water. Environ. Health Eng. Manag.. 2022; 9 (3) :305-310
URL: http://ehemj.com/article-1-1006-en.html

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Volume 9, Issue 3 (Summer 2022) Back to browse issues page
Environmental Health Engineering And Management Journal Environmental Health Engineering And Management Journal
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