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Cultivation of Chlorella pyrenoidosa as a raw material for the production of biofuels in palm oil mill effluent medium with the addition of urea and triple super phosphate
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Shinta Elystia    , Sri Rezeki Muria , Herta Furaida Erlangga |
| Corresponding author:Department of Environmental Engineering, Faculty of Engineering, Riau University, Kampus Bina Widya, Pekanbaru, Riau 28293, Indonesia , shintaelystia@yahoo.com |
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Abstract: (4767 Views) |
Background: The utilization of microalgae as a renewable energy is an important aspect in solving shortage of future oil reserve in 15 years. One of the renewable energy sources is microalgae biodiesel. Palm oil mill effluent (POME) is a wastewater that has a high content of organic materials. These organic materials can be used as growth nutrients for microalgae. Chlorella pyrenoidosa is one of the most potential microalgae used as a raw material for the production of biodiesel since it contains lipids (8%-35%).
Methods: Chlorella pyrenoidosa was cultured on the POME medium with concentrations of 0%, 25%, 50%, 75%, and 100%v with addition of synthetic nutrients (urea: TSP) at the ratios of 2:1 ; 1:2 ; 0.5:1 in a 500 mL Erlenmeyer flask, at pH 6-8, aeration using aquarium pumps, and using LED lights (3000 lux).
Results: It was revealed that at POME concentration of 25% and with addition of urea: TSP at a ratio of 2:1, the optimum specific growth rate (0.306/day) with the highest number of cells was 3.530 × 107 cells/mL and the highest lipid content was 36% of its dry weight. The removal efficiency of POME could be obtained from the removal efficiency of chemical oxygen demand (COD), total nitrogen, and orthophosphate (P-PO4), which was 70, 90.42, and 81.12%, respectively.
Conclusion: According to the results, under appropriate culture conditions, C. pyrenoidosa can produce lipids with good use of nutrients contained in the POME medium. |
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Keywords: Chlorella pyrenoidosa, POME, Lipid, Urea: TSP
eprint link: http://eprints.kmu.ac.ir/id/eprint/32336 |
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Full-Text [PDF 469 kb]
(1920 Downloads)
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Type of Study: Original Article |
Subject:
Special
Received: 2020/03/9 | Accepted: 2020/03/9 | Published: 2020/03/9
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